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Maize Transposable Element Database

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Impact of mothers' early life exposure to low or high folate on progeny outcome and DNA methylation patterns

Impact of mothers’ early life exposure to low or high folate on progeny outcome and DNA methylation patterns

November 29, 2020 by Ronald Elliott

The dynamic patterning of DNA and histone methylation throughout oocyte growth presents a doubtlessly vulnerable time for epigenetic disruption due to early life environmental exposure of future moms. We investigated whether or not maternal exposure to folic acid poor and supplemented diets beginning in utero may have an effect on oocytes and trigger opposed developmental and epigenetic results in subsequent technology progeny. Female BALB/c mice (F0) had been positioned on one of 4 amino acid outlined diets for four weeks earlier than being pregnant and all through gestation and lactation: folic acid management (rodent really useful every day consumption; Ctrl), 7-fold folic acid poor, 10-fold folic acid supplemented or 20-fold folic acid supplemented diets.

F1 feminine pups had been weaned onto Ctrl diets, mated to produce the F2 technology and the F2 offspring had been examined at E18.5 for developmental and epigenetic abnormalities. Resorption charges had been elevated and litter sizes decreased amongst F2 E18.5-day litters within the 20-fold folic acid supplemented group. Increases in irregular embryo outcomes had been noticed in all three folic acid poor and supplemented teams. Subtle genome-wide DNA methylation alterations had been discovered within the placentas and brains of F2 offspring within the 7-fold folic acid poor , 10-fold folic acid supplemented and 20-fold folic acid supplemented teams; in distinction, world and imprinted gene methylation weren’t affected.

The findings present that early life feminine environmental exposures to each low and high folate prior to oocyte maturation can compromise oocyte high quality, adversely affecting offspring of the subsequent technology, partly by altering DNA methylation patterns.

 

DNA methylation-based lung adenocarcinoma subtypes can predict prognosis, recurrence, and immunotherapeutic implications

 

The marked heterogeneity of lung adenocarcinoma (LUAD) makes its prognosis and therapy tough. In addition, the aberrant DNA methylation profile contributes to tumor heterogeneity and alters the immune response. We used DNA methylation array knowledge from publicly accessible databases to set up a predictive mannequin for LUAD prognosis.

Thirty-three methylation websites had been recognized as particular prognostic biomarkers, impartial of sufferers’ scientific traits. These methylation profiles had been used to establish potential drug candidates and examine the immune microenvironment of LUAD and response to immunotherapy. When in contrast with the high-risk group, the low-risk group had a decrease recurrence charge and favorable prognosis. The tumor microenvironment differed between the 2 teams as mirrored by the upper quantity of resting dendritic cells and a decrease quantity of monocytes and resting mast cells within the low-risk group.

Moreover, low-risk sufferers reported larger immune and stromal scores, decrease tumor purity, and larger expression of HLA genes. Low-risk sufferers responded properly to immunotherapy due to larger expression of immune checkpoint molecules and decrease stemness index. Thus, our mannequin predicted a positive prognosis and elevated total survival for sufferers within the low-risk methylation group. Further, this mannequin may present potential drug targets to develop efficient immunotherapies for LUAD.

Impact of mothers' early life exposure to low or high folate on progeny outcome and DNA methylation patterns
Impact of mothers’ early life exposure to low or high folate on progeny outcome and DNA methylation patterns

Deconvolution of mobile subsets in human tissue based mostly on focused DNA methylation evaluation at particular person CpG websites

Background: The complicated composition of totally different cell sorts inside a tissue might be estimated by deconvolution of bulk gene expression profiles or with numerous single-cell sequencing approaches. Alternatively, DNA methylation (DNAm) profiles have been used to set up an atlas for a number of human tissues and cell sorts. DNAm is especially appropriate for deconvolution of cell sorts as a result of every CG dinucleotide (CpG web site) has solely two states per DNA strand-methylated or non-methylated-and these epigenetic modifications are very constant throughout mobile differentiation. So far, deconvolution of DNAm profiles implies complicated signatures of many CpGs which are typically measured by genome-wide evaluation with Illumina BeadChip microarrays. In this examine, we investigated if the characterization of cell sorts in tissue can be possible with particular person cell type-specific CpG websites, which might be addressed by focused evaluation, akin to pyrosequencing.
Results: We compiled and curated 579 Illumina 450ok BeadChip DNAm profiles of 14 totally different non-malignant human cell sorts. A coaching and validation technique was utilized to establish and take a look at for cell type-specific CpGs. We initially targeted on estimating the relative quantity of fibroblasts utilizing two CpGs that had been both hypermethylated or hypomethylated in fibroblasts. The mixture of these two DNAm ranges right into a “FibroScore” correlated with the state of fibrosis and was related to total survival in numerous sorts of most cancers. Furthermore, we recognized hypomethylated CpGs for leukocytes, endothelial cells, epithelial cells, hepatocytes, glia, neurons, fibroblasts, and induced pluripotent stem cells. The accuracy of this eight CpG signature was examined in further BeadChip datasets of outlined cell mixtures and the outcomes had been comparable to beforehand revealed signatures based mostly on a number of thousand CpGs. Finally, we established and validated pyrosequencing assays for the related CpGs that may be utilized for classification and deconvolution of cell sorts.

DNA methylation inhibitor, Dichlone

2860-500 Biovision
EUR 109

DNA Methylation Detection Kit

K5082100 Biochain 1 kit
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Zebularine (DNA methylation inhibitor)

SIH-560-25MG Stressmarq 25 mg
EUR 311
Description: The substance Zebularine is a dna methylation inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (20 mg/ml); or water (20 mg/ml).

Zebularine (DNA methylation inhibitor)

SIH-560-5MG Stressmarq 5 mg
EUR 126
Description: The substance Zebularine is a dna methylation inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (20 mg/ml); or water (20 mg/ml).

Epizen DNA Methylation Kit

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Epizen DNA Methylation Kit

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EpiArt DNA Methylation Library Kit

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Epizen DNA Methylation-Gold Kit

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DNA Methylation Antibody Panel Pack I

C20000 EpiGentek
EUR 382.05
Description: Ask the seller for details

Methylamp Global DNA Methylation Quantification Ultra Kit

P-1014B EpiGentek 96 Assays
EUR 947.55
Description: reagents widely cited

Methyflash m6A DNA Methylation ELISA Kit (Colorimetric)

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  • Product category: Quality control/standard for molecular biology applications
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M-DNA-100BP CORNING 1/pk
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Description: Bioscience Mol Bio; DNA Ladder

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Description: Bioscience Mol Bio; DNA Ladder

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Description: Ask the seller for details

Global DNA Methylation ELISA Kit (5?-methyl-2?-deoxycytidine Quantitation)

STA-380 Cell Biolabs 96 assays
EUR 624
Description: The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

Global DNA Methylation ELISA Kit (5?-methyl-2?-deoxycytidine Quantitation)

STA-380-5 Cell Biolabs 5 x 96 assays
EUR 2503
Description: The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

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EUR 607

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Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE isotype controls (set of 7 IgGs)

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Histone H3K4 Methylation Antibody Panel Pack

C10005 EpiGentek 3 x 25 µg
EUR 628.55
Description: Ask the seller for details

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Description: kits suitable for this type of research

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Description: The best epigenetics products

Histone H3R2 Methylation Antibody Panel Pack

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Description: Ask the seller for details

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Description: kits suitable for this type of research

BSA Standard Solution

AJ642 BBI Biotech 1ml
EUR 60.44
  • Product category: Proteins/Protein Standards

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C001-500UL Arbor Assays 500UL
EUR 123

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EUR 123

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EUR 237

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EUR 123

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EUR 227

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EUR 123

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EUR 122

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EUR 123

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EUR 123

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EUR 123

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EUR 227

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EUR 123

Corticosterone Standard, 625UL

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EUR 227

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C046-225UL Arbor Assays 225UL
EUR 123

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C051-225UL Arbor Assays 225UL
EUR 123

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C054-125UL Arbor Assays 125UL
EUR 227

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EUR 123

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C054-625UL Arbor Assays 625UL
EUR 227

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EUR 143

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EUR 123

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C085-625UL Arbor Assays 625UL
EUR 237

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EUR 123

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EUR 123

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EUR 123

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EUR 237

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EUR 158

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C095-625UL Arbor Assays 625UL
EUR 308

Estradiol Standard, 125UL

C103-125UL Arbor Assays 125UL
EUR 123

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C103-625UL Arbor Assays 625UL
EUR 237

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EUR 123

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C110-625UL Arbor Assays 625UL
EUR 237

Testosterone Standard, 350UL

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EUR 237

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EUR 123

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EUR 123

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EUR 123

Prolactin Standard, 1EA

C139-1EA Arbor Assays 1EA
EUR 143

Glucose Standard, 90UL

C141-90UL Arbor Assays 90UL
EUR 123

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C146-90UL Arbor Assays 90UL
EUR 123

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C151-125UL Arbor Assays 125UL
EUR 123

Corticosterone Standard, 625UL

C151-625UL Arbor Assays 625UL
EUR 227

Allopregnanolone Standard, 125UL

C155-125UL Arbor Assays 125UL
EUR 123

Allopregnanolone Standard, 625UL

C155-625UL Arbor Assays 625UL
EUR 227

Estradiol Standard, 375UL

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EUR 227

Estradiol Standard, 75UL

C158-75UL Arbor Assays 75UL
EUR 122

Oxytocin Standard, 125UL

C164-125UL Arbor Assays 125UL
EUR 123

Oxytocin Standard, 625UL

C164-625UL Arbor Assays 625UL
EUR 237

Oxytocin Standard, 125UL

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EUR 123

Oxytocin Standard, 625UL

C167-625UL Arbor Assays 625UL
EUR 227

Thyroxine Standard, 200UL

C177-200UL Arbor Assays 200UL
EUR 227

Thyroxine Standard, 40UL

C177-40UL Arbor Assays 40UL
EUR 123

Aldosterone Standard, 125UL

C182-125UL Arbor Assays 125UL
EUR 123

Aldosterone Standard, 625UL

C182-625UL Arbor Assays 625UL
EUR 227

Ceruloplasmin Standard, 20UL

C189-20UL Arbor Assays 20UL
EUR 163

Levonorgestrel Standard, 350UL

C219-350UL Arbor Assays 350UL
EUR 227

Levonorgestrel Standard, 70UL

C219-70UL Arbor Assays 70UL
EUR 123

Myeloperoxidase Standard, 70UL

C225-70UL Arbor Assays 70UL
EUR 123

Epiandrosterone Standard, 125UL

C233-125UL Arbor Assays 125UL
EUR 123

Epiandrosterone Standard, 625UL

C233-625UL Arbor Assays 625UL
EUR 227

Estriol Standard, 125UL

C236-125UL Arbor Assays 125UL
EUR 123

Estriol Standard, 625UL

C236-625UL Arbor Assays 625UL
EUR 227

Progesterone Standard, 200UL

C252-200UL Arbor Assays 200UL
EUR 237

Progesterone Standard, 40UL

C252-40UL Arbor Assays 40UL
EUR 123

PKA Standard, 1EA

C131-1EA Arbor Assays 1EA
EUR 308

CHO HCP Standard

F018G Cygnus Technologies 1 ml
EUR 257
  • Product category: Quality control/standard for molecular biology applications
Description: CHO HCP Standard by Cygnus Technologies is available in Europe via Gentaur.

BSA Standard E

F031E Cygnus Technologies 1 ml
EUR 195
  • Product category: Quality control/standard for molecular biology applications
Description: BSA Standard E by Cygnus Technologies is available in Europe via Gentaur.

Insulin Standard G

F043G Cygnus Technologies 1 ml
EUR 195
  • Product category: Quality control/standard for molecular biology applications
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ExoStepTM Culture + Standard

ExoS-25-CST9 ImmunoStep 25 test
EUR 726

ExoStepTM Plasma + Standard

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ExoStepTM Urine + Standard

ExoS-25-UST9 ImmunoStep 25 test
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Standard Diluent Buffer

abx098951-20ml Abbexa 20 ml
EUR 91
  • Shipped within 5-7 working days.

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Peptide Standard 1

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Internal Standard R46594

50R-R46594 Fitzgerald 50 mg
EUR 241
Description: Internal standard 3-(2-[4-(4-chlorobenzyl)-1-piperidinyl]ethyl)-2,4-[1h,3H]-quinazoline-dione (R46594)
Conclusion: This proof of idea examine demonstrates that DNAm evaluation at particular person CpGs displays the mobile composition of mobile mixtures and totally different tissues. Targeted evaluation of these genomic areas facilitates strong strategies for utility in primary analysis and scientific settings.
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Conserved role of intragenic DNA methylation in regulating alternative promoters.
Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients

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Recent Posts

  • Investigating the Relationship Between Neuronal Cell Death and Early DNA Methylation After Ischemic Injury
  • The immune and metabolic changes with age in giant panda blood by combined transcriptome and DNA methylation analysis
  • Rare genetic variation at transcription factor binding sites modulates local DNA methylation profiles
  • DNA methylation changes in response to ocean acidification at the time of larval metamorphosis in the edible oyster, Crassostrea hongkongensis
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