Cusabio N-terminal 6xHis-Trx-tagged Recombinant

Product name

N-terminus 6xHis-Trx-tag, negative control

Full product name

N-terminal tag 6xHis-Trx

For research use only

For research use only. It should not be used in diagnostic procedures.

Host: E. coli

ISO certification

Manufactured in an ISO 9001:2015 certified laboratory.

Alternative name: TRX

Recommended dilutions/conditions:

  • Activity assay (>300U/mg)
  • Western Blot (100 ng, colourimetric)
  • Suggested dilutions/conditions may not be available for all applications.
  • Optimum conditions must be determined individually for each application.

Scientific Background:

N-terminal 6xHis Thioredoxin (Trx) tagged recombinant is a redox protein of approximately 12 kDa. Its major domain is conserved in several members of the Trx family and contains a conserved Cys-Gly-Pro-Cys catalytic site. It is ubiquitous and found in many organisms, from bacteria to mammals. Trx has been shown to function in cell proliferation, redox signalling, and inhibition of apoptosis.

Trx is reduced by the NADPH-dependent flavoenzyme thioredoxin reductase, and in turn, reduces other proteins such as protein disulfide isomerase (PDI) via cysteine ​​thiol-disulfide exchange. Trx has been reported to selectively activate the DNA binding of various transcription factors such as p53, NFkB, AP1, and the glucocorticoid receptor, resulting in altered gene transcription. Reduced Trx can provide reducing equivalents for ribonucleotide reductase, the essential enzyme for DNA synthesis, and thioredoxin peroxidase. As a cofactor for peroxiredoxins, Trx plays a direct role in the reduction of oxidative species such as H2O2.

PM: ~12kDa

Source: Produced in E. coli. Thioredoxin from E. coli is fused at the N-terminus with a His-tag.

Formulation: Liquid. In 50 mM TRIS, pH 7.5, containing 100 mM sodium chloride and 1 mM EDTA.

Purity: ≥95% (SDS-PAGE; Western blot)

Purity detail: Purified by multi-step chromatography.

Applications:

  • WB
  • activity assay

Application Notes: Western Blot Control.

Shipping: Shipped on dry ice

Long term storage: -80°C

Other notes

On occasion, small volumes of 6xHis-Trx-tag N-terminal Negative Control vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe the intended use will not meet our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice.