MTHFR C677T genetic polymorphism in combination with serum vitamin B2, B12 and aberrant DNA methylation of P16 and P53 genes in esophageal squamous cell carcinoma and esophageal precancerous lesions: a case-control study.

BackgroundThe research aimed to discover the associations between the interactions of serum vitamin B₂ or B₁₂ ranges, aberrant DNA methylation of p16 or p53 and MTHFR C677T polymorphism and the dangers of esophageal squamous cell carcinoma (ESCC) and esophageal precancerous lesion (EPL).

Methods200 ESCC instances, 200 EPL instances and 200 regular controls had been matched by age (± 2 years) and gender.

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Serum vitamin B₂ and B₁₂ ranges, MTHFR C677T genetic polymorphisms and the methylation standing of genes had been assessed. Chi sq. check, one-way evaluation of variance and binary logistic regression had been carried out.

ResultsThe lowest quartile of each serum vitamin B₂ and B₁₂ with TT genotype confirmed vital elevated EPL threat (OR = 4.91, 95% CI 1.31-18.35; OR = 6.88, 95% CI 1.10-42.80). The highest quartile of each serum vitamin B₂ and B₁₂ with CC genotype confirmed vital decreased ESCC threat (OR = 0.16, 95% CI 0.04-0.60; OR = 0.10, 95% CI 0.02-0.46).

The ORs of p16 methylation for genotype CT and TT had been 1.98 (95% CI 1.01-3.89) and 17.79 (95% CI 2.26-140.22) in EPL, 4.86 (95% CI 2.48-9.50) and 20.40 (95% CI 2.53-164.81) in ESCC, respectively. Similarly, p53 methylation with genotype TT was related with elevated EPL and ESCC dangers (OR = 13.28, 95% CI 1.67-105.70; OR = 15.24, 95% CI 1.90-122.62).

ConclusionsThe MTHFR C677T genotype and serum vitamin B₂ or B₁₂ ranges could work together in methods which related with the EPL and ESCC dangers. The gene-gene interplay advised that aberrant DNA methyaltion of both p16 or p53 mixed with T alleles of MTHFR was related with elevated dangers of each EPL and ESCC.