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Maize Transposable Element Database

Maize Transposable Element Database

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Impact of mothers' early life exposure to low or high folate on progeny outcome and DNA methylation patterns

Impact of mothers’ early life exposure to low or high folate on progeny outcome and DNA methylation patterns

November 29, 2020 by Ronald Elliott

The dynamic patterning of DNA and histone methylation throughout oocyte growth presents a doubtlessly vulnerable time for epigenetic disruption due to early life environmental exposure of future moms. We investigated whether or not maternal exposure to folic acid poor and supplemented diets beginning in utero may have an effect on oocytes and trigger opposed developmental and epigenetic results in subsequent technology progeny. Female BALB/c mice (F0) had been positioned on one of 4 amino acid outlined diets for four weeks earlier than being pregnant and all through gestation and lactation: folic acid management (rodent really useful every day consumption; Ctrl), 7-fold folic acid poor, 10-fold folic acid supplemented or 20-fold folic acid supplemented diets.

F1 feminine pups had been weaned onto Ctrl diets, mated to produce the F2 technology and the F2 offspring had been examined at E18.5 for developmental and epigenetic abnormalities. Resorption charges had been elevated and litter sizes decreased amongst F2 E18.5-day litters within the 20-fold folic acid supplemented group. Increases in irregular embryo outcomes had been noticed in all three folic acid poor and supplemented teams. Subtle genome-wide DNA methylation alterations had been discovered within the placentas and brains of F2 offspring within the 7-fold folic acid poor , 10-fold folic acid supplemented and 20-fold folic acid supplemented teams; in distinction, world and imprinted gene methylation weren’t affected.

The findings present that early life feminine environmental exposures to each low and high folate prior to oocyte maturation can compromise oocyte high quality, adversely affecting offspring of the subsequent technology, partly by altering DNA methylation patterns.

 

DNA methylation-based lung adenocarcinoma subtypes can predict prognosis, recurrence, and immunotherapeutic implications

 

The marked heterogeneity of lung adenocarcinoma (LUAD) makes its prognosis and therapy tough. In addition, the aberrant DNA methylation profile contributes to tumor heterogeneity and alters the immune response. We used DNA methylation array knowledge from publicly accessible databases to set up a predictive mannequin for LUAD prognosis.

Thirty-three methylation websites had been recognized as particular prognostic biomarkers, impartial of sufferers’ scientific traits. These methylation profiles had been used to establish potential drug candidates and examine the immune microenvironment of LUAD and response to immunotherapy. When in contrast with the high-risk group, the low-risk group had a decrease recurrence charge and favorable prognosis. The tumor microenvironment differed between the 2 teams as mirrored by the upper quantity of resting dendritic cells and a decrease quantity of monocytes and resting mast cells within the low-risk group.

Moreover, low-risk sufferers reported larger immune and stromal scores, decrease tumor purity, and larger expression of HLA genes. Low-risk sufferers responded properly to immunotherapy due to larger expression of immune checkpoint molecules and decrease stemness index. Thus, our mannequin predicted a positive prognosis and elevated total survival for sufferers within the low-risk methylation group. Further, this mannequin may present potential drug targets to develop efficient immunotherapies for LUAD.

Impact of mothers' early life exposure to low or high folate on progeny outcome and DNA methylation patterns
Impact of mothers’ early life exposure to low or high folate on progeny outcome and DNA methylation patterns

Deconvolution of mobile subsets in human tissue based mostly on focused DNA methylation evaluation at particular person CpG websites

Background: The complicated composition of totally different cell sorts inside a tissue might be estimated by deconvolution of bulk gene expression profiles or with numerous single-cell sequencing approaches. Alternatively, DNA methylation (DNAm) profiles have been used to set up an atlas for a number of human tissues and cell sorts. DNAm is especially appropriate for deconvolution of cell sorts as a result of every CG dinucleotide (CpG web site) has solely two states per DNA strand-methylated or non-methylated-and these epigenetic modifications are very constant throughout mobile differentiation. So far, deconvolution of DNAm profiles implies complicated signatures of many CpGs which are typically measured by genome-wide evaluation with Illumina BeadChip microarrays. In this examine, we investigated if the characterization of cell sorts in tissue can be possible with particular person cell type-specific CpG websites, which might be addressed by focused evaluation, akin to pyrosequencing.
Results: We compiled and curated 579 Illumina 450ok BeadChip DNAm profiles of 14 totally different non-malignant human cell sorts. A coaching and validation technique was utilized to establish and take a look at for cell type-specific CpGs. We initially targeted on estimating the relative quantity of fibroblasts utilizing two CpGs that had been both hypermethylated or hypomethylated in fibroblasts. The mixture of these two DNAm ranges right into a “FibroScore” correlated with the state of fibrosis and was related to total survival in numerous sorts of most cancers. Furthermore, we recognized hypomethylated CpGs for leukocytes, endothelial cells, epithelial cells, hepatocytes, glia, neurons, fibroblasts, and induced pluripotent stem cells. The accuracy of this eight CpG signature was examined in further BeadChip datasets of outlined cell mixtures and the outcomes had been comparable to beforehand revealed signatures based mostly on a number of thousand CpGs. Finally, we established and validated pyrosequencing assays for the related CpGs that may be utilized for classification and deconvolution of cell sorts.

Zebularine (DNA methylation inhibitor)

SIH-560-25MG Stressmarq 25 mg
EUR 311
Description: The substance Zebularine is a dna methylation inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (20 mg/ml); or water (20 mg/ml).

Zebularine (DNA methylation inhibitor)

SIH-560-5MG Stressmarq 5 mg
EUR 126
Description: The substance Zebularine is a dna methylation inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is white powder which is May be dissolved in DMSO (20 mg/ml); or water (20 mg/ml).

Epizen DNA Methylation Kit

Z4201-050 GenDepot 50 Rxns
EUR 327

Epizen DNA Methylation Kit

Z4201-200 GenDepot 4x50 rxns
EUR 968

EpiArt DNA Methylation Bisulfite Kit

EM101-01 Vazyme 50 rxn
EUR 241

EpiArt DNA Methylation Bisulfite Kit

EM101-02 Vazyme 200 rxn
EUR 609

EpiArt DNA Methylation Library Kit

NE101-01 Vazyme 24 rxn
EUR 889

EpiArt DNA Methylation Library Kit

NE101-02 Vazyme 96 rxn
EUR 2928

Epizen DNA Methylation-Direct Kit

Z4001-050 GenDepot 50 Rxns
EUR 428

Epizen DNA Methylation-Direct Kit

Z4001-200 GenDepot 4x50 rxns
EUR 1036

Epizen DNA Methylation-Gold Kit

Z4101-050 GenDepot 50 Rxns
EUR 361

Epizen DNA Methylation-Gold Kit

Z4101-200 GenDepot 4x50 rxns
EUR 901

DNA Methylation Antibody Panel Pack I

C20000 EpiGentek
EUR 382.05
Description: Ask the seller for details

Methylamp Global DNA Methylation Quantification Ultra Kit

P-1014B EpiGentek 96 Assays
EUR 947.55
Description: reagents widely cited

Methyflash m6A DNA Methylation ELISA Kit (Colorimetric)

P-9010 EpiGentek 96 Assays
EUR 788.05
Description: The best epigenetics products

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP CORNING 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB CORNING 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

P-1032 EpiGentek 96 reactions
EUR 889.55
Description: The best epigenetics products

Human DNA Standard (A-G)

D163 Cygnus Technologies 1 ml
EUR 322
Description: Human DNA Standard (A-G) by Cygnus Technologies is available in Europe via Gentaur.

Set of 10 Biolipidure Reagents

Biolipidure-set Biolipidure 10mLx10
EUR 1517
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Global DNA Methylation ELISA Kit (5?-methyl-2?-deoxycytidine Quantitation)

STA-380 Cell Biolabs 96 assays
EUR 624
Description: The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

Global DNA Methylation ELISA Kit (5?-methyl-2?-deoxycytidine Quantitation)

STA-380-5 Cell Biolabs 5 x 96 assays
EUR 2503
Description: The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

P-1030 EpiGentek 96 reactions
EUR 860.55
Description: Ask the seller for details

StemBoost? YPAC Cocktail Set (1000X), Sterile-Filtered

K871-set Biovision
EUR 958

StemBoost? Reprogramming Cocktail Set I (1000X), Sterile-Filtered

K869-set Biovision
EUR 849

StemBoost? Reprogramming Cocktail Set II (1000X), Sterile-Filtered

K870-set Biovision
EUR 838

StemBoost? 2i-Reprogramming Cocktail Set (1000X), Sterile-Filtered

K889-set Biovision
EUR 620

StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered

K877-set Biovision
EUR 512

StemBoost? Neuronal Cell Induction Cocktail Set (100X), Sterile-Filtered

K891-set Biovision
EUR 805

Antigen-Antibody Pens, a set of any 3 pens

PEN13-SET Alpha Diagnostics 3
EUR 451

Standard

abx296006-1vial Abbexa 1 vial
EUR 300

Standard

abx092106-1vial Abbexa 1 vial
EUR 217

Standard

abx098954-10vials Abbexa 10 vials
EUR 2179

Standard

abx098954-1vial Abbexa 1 vial
EUR 300

Standard

abx098954-5vials Abbexa 5 vials
EUR 1177

Standard

abx098955-10vials Abbexa 10 vials
EUR 2179

Standard

abx098955-1vial Abbexa 1 vial
EUR 300

Standard

abx098955-5vials Abbexa 5 vials
EUR 1177

Standard

abx098956-10vials Abbexa 10 vials
EUR 2179

Standard

abx098956-1vial Abbexa 1 vial
EUR 300

Standard

abx098956-5vials Abbexa 5 vials
EUR 1177

Standard

abx098963-10vials Abbexa 10 vials
EUR 2179

Standard

abx098963-1vial Abbexa 1 vial
EUR 300

Standard

abx098963-5vials Abbexa 5 vials
EUR 1177

Standard

abx098965-10vials Abbexa 10 vials
EUR 2179

Standard

abx098965-1vial Abbexa 1 vial
EUR 300

Standard

abx098965-5vials Abbexa 5 vials
EUR 1177

Standard

abx098967-10vials Abbexa 10 vials
EUR 2179

Standard

abx098967-1vial Abbexa 1 vial
EUR 300

Standard

abx098967-5vials Abbexa 5 vials
EUR 1177

Standard

abx098968-10vials Abbexa 10 vials
EUR 2179

Standard

abx098968-1vial Abbexa 1 vial
EUR 300

Standard

abx098968-5vials Abbexa 5 vials
EUR 1177

Standard

abx098969-10vials Abbexa 10 vials
EUR 2179

Standard

abx098969-1vial Abbexa 1 vial
EUR 300

Standard

abx098969-5vials Abbexa 5 vials
EUR 1177

Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE isotype controls (set of 7 IgGs)

20102-SET Alpha Diagnostics 1 Set (100 ugx7)
EUR 598

Deoxynivalenol Standard

SD010 Unibiotest 0.5ug/mL
EUR 607

Zearalenone Standard

SD013 Unibiotest 0.5ug/mL
EUR 523

Tetrodotoxin Standard

SD015 Unibiotest 0.5ug/mL
EUR 607

Microcystin Standard

SD016 Unibiotest 0.5ug/mL
EUR 523

Histone H3K4 Methylation Antibody Panel Pack

C10005 EpiGentek 3 x 25 µg
EUR 628.55
Description: Ask the seller for details

Histone H3K9 Methylation Antibody Panel Pack

C10006 EpiGentek 3 x 25 µg
EUR 628.55
Description: fast delivery possible

Histone H3K27 Methylation Antibody Panel Pack

C10007 EpiGentek 3 x 25 µg
EUR 628.55
Description: The best epigenetics products

Histone H3K36 Methylation Antibody Panel Pack

C10008 EpiGentek 3 x 25 µg
EUR 628.55
Description: reagents widely cited

Histone H3K79 Methylation Antibody Panel Pack

C10009 EpiGentek 3 x 25 µg
EUR 628.55
Description: kits suitable for this type of research

Histone H4K20 Methylation Antibody Panel Pack

C10012 EpiGentek 3 x 25 µg
EUR 628.55
Description: The best epigenetics products

Histone H3R2 Methylation Antibody Panel Pack

C10015 EpiGentek 3 x 25 µg
EUR 628.55
Description: Ask the seller for details

Histone H3R8 Methylation Antibody Panel Pack

C10016 EpiGentek 3 x 25 µg
EUR 628.55
Description: fast delivery possible

Histone H3R17 Methylation Antibody Panel Pack

C10017 EpiGentek 3 x 25 µg
EUR 628.55
Description: The best epigenetics products

Histone H3R26 Methylation Antibody Panel Pack

C10018 EpiGentek 3 x 25 µg
EUR 628.55
Description: reagents widely cited

Histone H4R3 Methylation Antibody Panel Pack

C10019 EpiGentek 3 x 25 µg
EUR 628.55
Description: kits suitable for this type of research

BSA (Standard Grade)

30-AB70 Fitzgerald 1 kg
EUR 899
Description: Standard Grade Bovine Serum Albumin (99% pure)

Internal Standard R46594

50R-R46594 Fitzgerald 50 mg
EUR 241
Description: Internal standard 3-(2-[4-(4-chlorobenzyl)-1-piperidinyl]ethyl)-2,4-[1h,3H]-quinazoline-dione (R46594)

Itraconazole Internal Standard

50R-R51012 Fitzgerald 50 mg
EUR 241
Description: Itraconazole chemical reference substance

Itraconazole Reference Standard

50R-R51211 Fitzgerald 50 mg
EUR 133
Description: Itraconazole chemical reference substance

Formaldehyde Standard, 500UL

C001-500UL Arbor Assays 500UL
EUR 123

Creatinine Standard, 100UL

C003-100UL Arbor Assays 100UL
EUR 123

Creatinine Standard, 1ML

C003-1ML Arbor Assays 1ML
EUR 237

RBP Standard, 60UL

C009-60UL Arbor Assays 60UL
EUR 123

RBP Standard, 240UL

C014-240UL Arbor Assays 240UL
EUR 227

RBP Standard, 60UL

C014-60UL Arbor Assays 60UL
EUR 123

Palladium Standard, 100UL

C016-100UL Arbor Assays 100UL
EUR 123

Glutathione Standard, 100UL

C018-100UL Arbor Assays 100UL
EUR 122

Glutathione Standard, 300UL

C018-300UL Arbor Assays 300UL
EUR 123

Hemoglobin Standard, 300UL

C037-300UL Arbor Assays 300UL
EUR 123

Cortisol Standard, 125UL

C040-125UL Arbor Assays 125UL
EUR 123

Cortisol Standard, 625UL

C040-625UL Arbor Assays 625UL
EUR 227

Corticosterone Standard, 125UL

C043-125UL Arbor Assays 125UL
EUR 123

Corticosterone Standard, 625UL

C043-625UL Arbor Assays 625UL
EUR 227

Acetylcholinesterase Standard, 225UL

C046-225UL Arbor Assays 225UL
EUR 123

Butyrylcholinesterase Standard, 225UL

C051-225UL Arbor Assays 225UL
EUR 123

Cortisone Standard, 125UL

C054-125UL Arbor Assays 125UL
EUR 227

Cortisone Standard, 50UL

C054-50UL Arbor Assays 50UL
EUR 123

Cortisone Standard, 625UL

C054-625UL Arbor Assays 625UL
EUR 227

Osteopontin Standard, 1EA

C077-1EA Arbor Assays 1EA
EUR 143

PGFM Standard, 125UL

C085-125UL Arbor Assays 125UL
EUR 123

PGFM Standard, 625UL

C085-625UL Arbor Assays 625UL
EUR 237

Nitrate Standard, 200UL

C086-200UL Arbor Assays 200UL
EUR 123

Nitrite Standard, 200UL

C087-200UL Arbor Assays 200UL
EUR 123

Progesterone Standard, 125UL

C092-125UL Arbor Assays 125UL
EUR 123

Progesterone Standard, 625UL

C092-625UL Arbor Assays 625UL
EUR 237

ANP Standard, 125UL

C095-125UL Arbor Assays 125UL
EUR 158

ANP Standard, 625UL

C095-625UL Arbor Assays 625UL
EUR 308

Estradiol Standard, 125UL

C103-125UL Arbor Assays 125UL
EUR 123

Estradiol Standard, 625UL

C103-625UL Arbor Assays 625UL
EUR 237

Estrone Standard, 125UL

C110-125UL Arbor Assays 125UL
EUR 123

Estrone Standard, 625UL

C110-625UL Arbor Assays 625UL
EUR 237

Testosterone Standard, 350UL

C113-350UL Arbor Assays 350UL
EUR 237

Testosterone Standard, 70UL

C113-70UL Arbor Assays 70UL
EUR 123

Catalase Standard, 90UL

C114-90UL Arbor Assays 90UL
EUR 123

PKA Standard, 1EA

C131-1EA Arbor Assays 1EA
EUR 308

Glucose Standard, 90UL

C136-90UL Arbor Assays 90UL
EUR 123

Prolactin Standard, 1EA

C139-1EA Arbor Assays 1EA
EUR 143

Glucose Standard, 90UL

C141-90UL Arbor Assays 90UL
EUR 123

Galactose Standard, 90UL

C146-90UL Arbor Assays 90UL
EUR 123

Corticosterone Standard, 125UL

C151-125UL Arbor Assays 125UL
EUR 123

Corticosterone Standard, 625UL

C151-625UL Arbor Assays 625UL
EUR 227

Allopregnanolone Standard, 125UL

C155-125UL Arbor Assays 125UL
EUR 123

Allopregnanolone Standard, 625UL

C155-625UL Arbor Assays 625UL
EUR 227

Estradiol Standard, 375UL

C158-375UL Arbor Assays 375UL
EUR 227

Estradiol Standard, 75UL

C158-75UL Arbor Assays 75UL
EUR 122

Oxytocin Standard, 125UL

C164-125UL Arbor Assays 125UL
EUR 123

Oxytocin Standard, 625UL

C164-625UL Arbor Assays 625UL
EUR 237

Oxytocin Standard, 125UL

C167-125UL Arbor Assays 125UL
EUR 123

Oxytocin Standard, 625UL

C167-625UL Arbor Assays 625UL
EUR 227

Thyroxine Standard, 200UL

C177-200UL Arbor Assays 200UL
EUR 227

Thyroxine Standard, 40UL

C177-40UL Arbor Assays 40UL
EUR 123

Aldosterone Standard, 125UL

C182-125UL Arbor Assays 125UL
EUR 123

Aldosterone Standard, 625UL

C182-625UL Arbor Assays 625UL
EUR 227

Ceruloplasmin Standard, 20UL

C189-20UL Arbor Assays 20UL
EUR 163

Levonorgestrel Standard, 350UL

C219-350UL Arbor Assays 350UL
EUR 227

Levonorgestrel Standard, 70UL

C219-70UL Arbor Assays 70UL
EUR 123

Myeloperoxidase Standard, 70UL

C225-70UL Arbor Assays 70UL
EUR 123

Epiandrosterone Standard, 125UL

C233-125UL Arbor Assays 125UL
EUR 123

Epiandrosterone Standard, 625UL

C233-625UL Arbor Assays 625UL
EUR 227

Estriol Standard, 125UL

C236-125UL Arbor Assays 125UL
EUR 123

Estriol Standard, 625UL

C236-625UL Arbor Assays 625UL
EUR 227

Progesterone Standard, 200UL

C252-200UL Arbor Assays 200UL
EUR 237

Progesterone Standard, 40UL

C252-40UL Arbor Assays 40UL
EUR 123

STANDARD METHODS AGAR

S19-113-10kg Alphabiosciences 10 kg
EUR 1027

STANDARD METHODS AGAR

S19-113-2Kg Alphabiosciences 2 Kg
EUR 262

STANDARD METHODS AGAR

S19-113-500g Alphabiosciences 500 g
EUR 108

Peptide Standard 1

5-01747 CHI Scientific 4 x 1mg Ask for price

BSA Standard Solution

AJ642 Bio Basic 1ml
EUR 60.44

Standard Diluent Buffer

abx098951-20ml Abbexa 20 ml
EUR 91

Lyophilized recombinant standard

ST0000-1 BosterBio 1ng/vial
EUR 172

Lyophilized recombinant standard

ST0000-10 BosterBio 10ng/vial
EUR 172

Lyophilized recombinant standard

ST0000-100 BosterBio 100ng/vial
EUR 172

Lyophilized recombinant standard

ST0000-20 BosterBio 20ng/vial
EUR 172
Conclusion: This proof of idea examine demonstrates that DNAm evaluation at particular person CpGs displays the mobile composition of mobile mixtures and totally different tissues. Targeted evaluation of these genomic areas facilitates strong strategies for utility in primary analysis and scientific settings.
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Conserved role of intragenic DNA methylation in regulating alternative promoters.
Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients

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