Global profile of tRNA-derived small RNAs in gastric cancer patient plasma and identification of tRF-33-P4R8YP9LON4VDP as a new tumor suppressor

Global profile of tRNA-derived small RNAs in gastric cancer patient plasma and identification of tRF-33-P4R8YP9LON4VDP as a new tumor suppressor

Transfer RNA (tRNA)-derived small RNAs (tsRNAs) have been discovered to play vital roles in the incidence and improvement of cancers. However, the tsRNA profile in gastric cancer is unknown. In this examine, we aimed to determine the worldwide tsRNA profile in plasma from gastric cancer sufferers and elucidate the function of tRF-33-P4R8YP9LON4VDP in gastric cancer. … Read more

The immune and metabolic changes with age in giant panda blood by combined transcriptome and DNA methylation analysis

The immune and metabolic changes with age in giant panda blood by combined transcriptome and DNA methylation analysis

Giant panda (Ailuropoda melanoleuca) is an endangered mammalian species. Exploring immune and metabolic changes that happen in giant pandas with age is vital for his or her safety. In this examine, we systematically investigated the physiological and biochemical indicators in blood, in addition to the transcriptome, and methylation profiles of younger, grownup, and outdated giant … Read more

Rare genetic variation at transcription factor binding sites modulates local DNA methylation profiles

Rare genetic variation at transcription factor binding sites modulates local DNA methylation profiles

Although DNA methylation is one of the best characterised epigenetic mark, the mechanism by which it’s focused to particular areas within the genome stays unclear. Recent research have revealed that local DNA methylation profiles may be dictated by cis-regulatory DNA sequences that primarily function by way of DNA-binding components. Consistent with this discovering, we now … Read more

DNA methylation changes in response to ocean acidification at the time of larval metamorphosis in the edible oyster, Crassostrea hongkongensis

Introduction: Recently we have identified a novel RASSF1C-PIWIL1-piRNA pathway that promotes lung cancer cell progression and migration. PIWI-like proteins interact with piRNAs to form complexes that regulate gene expression at the transcriptional and translational levels. We have illustrated in previous work that RASSF1C modulates the expression of the PIWIL1-piRNA gene axis, suggesting the hypothesis that the RASSF1C-PIWI-piRNA pathway could potentially contribute to lung cancer stem cell development and progression, in part, through modulation of gene methylation of both oncogenic and tumor suppressor genes. Therefore, we tested this hypothesis using a non-small cell lung cancer (NSCLC) cell model to identify Candidate Differentially Methylated Regions (DMRs) modulated by the RASSF1C-PIWIL1-piRNA pathway. Materials and methods: We studied the impact of over-expressing RASSF1C and knocking down RASSF1C and PIWIL1 expression on global gene DNA methylation in the NSCLC cell line H1299 using the Reduced Representation Bisulfite Sequencing (RRBS) method. Results: DMRs were identified by comparing DNA methylation profiles of experimental and control cells. Over-expression of RASSF1C and knocking down RASSF1C and PIWIL1 modulated DNA methylation of genomic regions; and statistically significant candidate genes residing DMR regions in lung cancer cells were identified, including oncogenes and tumor suppressors. One of the hypermethylated genes, Gem Interacting Protein (GMIP), displays tumor suppressor properties. GMIP expression attenuates lung cancer cell migration, and its over-expression is associated with longer survival of lung cancer patients. Conclusions: The RASSF1C-PIWI-piRNA pathway modulates key oncogenes and tumor suppressor genes. GMIP is hypermethylated by this pathway and has tumor suppressor properties.

Unprecedented price of elevated CO2 degree in the ocean and the subsequent modifications in carbonate system including decreased pH, often called ocean acidification (OA), is predicted to disrupt not solely the calcification course of but additionally a number of different physiological and developmental processes in a selection of marine organisms, including edible oysters. Nonetheless, not all … Read more

Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients

Introduction: Recently we have identified a novel RASSF1C-PIWIL1-piRNA pathway that promotes lung cancer cell progression and migration. PIWI-like proteins interact with piRNAs to form complexes that regulate gene expression at the transcriptional and translational levels. We have illustrated in previous work that RASSF1C modulates the expression of the PIWIL1-piRNA gene axis, suggesting the hypothesis that the RASSF1C-PIWI-piRNA pathway could potentially contribute to lung cancer stem cell development and progression, in part, through modulation of gene methylation of both oncogenic and tumor suppressor genes. Therefore, we tested this hypothesis using a non-small cell lung cancer (NSCLC) cell model to identify Candidate Differentially Methylated Regions (DMRs) modulated by the RASSF1C-PIWIL1-piRNA pathway. Materials and methods: We studied the impact of over-expressing RASSF1C and knocking down RASSF1C and PIWIL1 expression on global gene DNA methylation in the NSCLC cell line H1299 using the Reduced Representation Bisulfite Sequencing (RRBS) method. Results: DMRs were identified by comparing DNA methylation profiles of experimental and control cells. Over-expression of RASSF1C and knocking down RASSF1C and PIWIL1 modulated DNA methylation of genomic regions; and statistically significant candidate genes residing DMR regions in lung cancer cells were identified, including oncogenes and tumor suppressors. One of the hypermethylated genes, Gem Interacting Protein (GMIP), displays tumor suppressor properties. GMIP expression attenuates lung cancer cell migration, and its over-expression is associated with longer survival of lung cancer patients. Conclusions: The RASSF1C-PIWI-piRNA pathway modulates key oncogenes and tumor suppressor genes. GMIP is hypermethylated by this pathway and has tumor suppressor properties.

Vitamin B12 has been broadly associated to methionine metabolism, which is an integral part for organic methylation reactions, together with DNA methylation. However, the connection between vitamin B12 and DNA methylation continues to be controversial. In addition, there’s rising proof for the affiliation between vitamin B12 and the danger of colorectal most cancers (CRC), though … Read more

Genome-scale DNA methylation maps of pluripotent and differentiated cells.

Genome-scale DNA methylation maps of pluripotent and differentiated cells.

DNA methylation is important for regular growth and has been implicated in lots of pathologies together with most cancers. Our information concerning the genome-wide distribution of DNA methylation, the way it modifications throughout mobile differentiation and the way it pertains to histone methylation and different chromatin modifications in mammals stays restricted. Here we report the … Read more