A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

by

Enterohemorrhagic Escherichia coli serotype O157:H7 (O157) is a crucial, foodborne, human intestinal pathogen that causes extreme acute hemorrhagic diarrhea, belly cramping, and even loss of life. Small RNAs (sRNAs) are noncoding regulatory molecules that sense environmental adjustments and set off numerous virulence-related signaling pathways; nonetheless, few such sRNAs have been recognized in O157. Here, we report a novel sRNA, EsrF that senses excessive ammonium concentrations in the colon and enhances O157 pathogenicity by selling bacterial motility and adhesion to host cells.

Specifically, EsrF was discovered to instantly work together with the 5′ untranslated areas of the flagellar biosynthetic gene, flhB, mRNA and improve its abundance, thereby upregulating expression of important flagellar genes, together with flhD, flhC, fliA, and fliC, main to elevated O157 motility and virulence. Meanwhile, an toddler rabbit mannequin of O157 an infection confirmed that deletion of esrF and flhB considerably attenuates O157 pathogenicity.

Furthermore, NtrC-the response regulator of the NtrC/B two-component system-was discovered to exert direct, damaging regulation of esrF expression. Meanwhile, excessive ammonium concentrations in the colon launch the inhibitory impact of NtrC on esrF, thereby enhancing its expression and subsequently selling bacterial colonization in the host colon. Our work reveals a novel, sRNA-centered, virulence-related signaling pathway in O157 that senses excessive ammonium concentrations. These findings present novel insights for future analysis on O157 pathogenesis and focused therapy methods.

The course of by which micro organism sense environmental cues to regulate their virulence is advanced. Several research have centered on regulating the expression of the locus of enterocyte effacement pathogenicity island in the everyday intestine pathogenic bacterium, O157. However, few investigations have addressed the regulation of different virulence elements in response to intestinal indicators. In this research, we report our discovery of a novel O157 sRNA, EsrF, and show that it contributed to bacterial motility and virulence in vitro and in vivo by the regulation of bacterial flagellar synthesis.

Furthermore, we present that top ammonium concentrations in the colon induced esrF expression to promote bacterial virulence by releasing the repression of esrF by NtrC. This research highlights the significance of sRNA in regulating the motility and pathogenicity of O157.  In this research, carboxyalkylated-PEI25 (25 kDa) was used to ship plasmids expressing SALL4-siRNA into MCF-7 cells. DLS and AFM had been utilized to decide the dimensions of nanoparticles. The MTT technique was used to assess cytotoxicity, and the effectivity of transfection was confirmed each qualitatively and quantitatively. Finally, the impact of silencing SALL4 was investigated on the migration of MCF7 cells utilizing the scratch check.

Polyploidy-associated paramutation in Arabidopsis is set by small RNAs, temperature, and allele construction

Paramutation is a kind of non-Mendelian inheritance in which the expression of a paramutable allele adjustments when it encounters a paramutagenic allele. This change in expression of the paramutable alleles is stably inherited even after segregation of each alleles. While the invention of paramutation and research of its underlying mechanism had been made with alleles that change plant pigmentation, paramutation-like phenomena are recognized to modulate the expression of different traits and in different eukaryotes, and many instances have most likely gone undetected.

It is probably going that epigenetic mechanisms are accountable for the phenomenon, as paramutation types epialleles, genes with similar sequences however totally different expression states. This might account for the intergenerational inheritance of the paramutated allele, offering profound proof that triggered epigenetic adjustments will be maintained over generations. Here, we use a case of paramutation that impacts a transgenic choice reporter gene in tetraploid Arabidopsis thaliana. Our information counsel that differing types of small RNA are derived from paramutable and paramutagenic epialleles.

In addition, deletion of a repeat inside the epiallele adjustments its paramutability. Further, the temperature through the progress of the epiallelic hybrids determines the diploma and timing of the allelic interplay. The information additional make it believable why paramutation in this method turns into evident solely in the segregating F2 inhabitants of tetraploid vegetation containing each epialleles. In abstract, the outcomes help a mannequin for polyploidy-associated paramutation, with similarities in addition to distinctions from different instances of paramutation.

A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

A artificial small molecule stalls pre-mRNA splicing by selling an early-stage U2AF2-RNA advanced

Dysregulated pre-mRNA splicing is an rising Achilles heel of cancers and myelodysplasias. To develop the at present restricted portfolio of small-molecule drug leads, we screened for chemical modulators of the U2AF advanced, which nucleates spliceosome meeting and is mutated in myelodysplasias. A hit compound particularly enhances RNA binding by a U2AF2 subunit. Remarkably, the compound inhibits splicing of consultant substrates and stalls spliceosome meeting on the stage of U2AF perform. Computational docking, along with structure-guided mutagenesis, signifies that the compound bridges the tandem U2AF2 RNA recognition motifs through hydrophobic and electrostatic moieties. Cells expressing a cancer-associated

Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) Antibody
abx238835-100ug 100 ug
EUR 509

ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE101020-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE101020-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE101020-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mouse Troponin C, slow skeletal and cardiac muscles, Tnnc1 ELISA
ELI-36461m 96 Tests
EUR 865

Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS
ELI-17222b 96 Tests
EUR 928

Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELI
ELI-39993p 96 Tests
EUR 928

Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA
ELI-40072h 96 Tests
EUR 824

Human TNNC1/ Troponin C, slow skeletal and cardiac muscles ELISA Kit
E2558Hu 1 Kit
EUR 605

Chicken Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555347-96tests 96 tests
EUR 911

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555435-96tests 96 tests
EUR 786

Mouse Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555608-96tests 96 tests
EUR 739

Pig Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555672-96tests 96 tests
EUR 911

Cow Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx555769-96tests 96 tests
EUR 911

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
abx352187-96tests 96 tests
EUR 786

Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit
20-abx153332
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA kit for Human Troponin C, slow skeletal and cardiac muscles
EK3670 96 tests
EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-48T 48T
EUR 425
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-5platesof96wells 5 plates of 96 wells
EUR 3026
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
KTE50026-96T 96T
EUR 756
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5 1
EUR 1138

Rat Skeletal Muscle Tissue Lysate
LYSATE0010 200ug
EUR 150
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Total Protein Extraction Kit for Muscles (50 tests)
P5A06 - Ask for price

Rat Skeletal Muscle Whole tissue lysate
RAL-1467 1 mg
EUR 524

rno-miRNome MicroRNA Profiling Kit [Rat]
RA680B-1 20 complete profiles
EUR 1672

Myosin antibody (skeletal)
10R-7875 100 ug
EUR 322
Description: Mouse monoclonal Myosin antibody

Myosin antibody (skeletal)
10R-7876 100 ug
EUR 322
Description: Mouse monoclonal Myosin antibody

Skeletal Muscle Lysate
1407 0.1 mg
EUR 191
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skeletal Muscle Lysate
1467 0.1 mg
EUR 191
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skeletal Muscle Lysate
21-107 0.1 mg
EUR 285.5
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Skeletal Muscle Lysate
21-197 0.1 mg
EUR 285.5
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Skeletal Muscle Lysate
1375 0.1 mg
EUR 191
Description: Skeletal Muscle tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

MicroRNA Isolation Kit
KS341025 1 kit
EUR 256
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells
2-82573 1 Kit Ask for price

Rat Ryanodine Receptor 1, Skeletal (RYR1) Protein
20-abx654972
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Troponin-I Rat Skeletal Muscle, pure protein
TNIM50-N-25 25 ug
EUR 225

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Skeletal muscle Actinin Alpha ELISA kit
E02S0267-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

cDNA from Rat Normal Tissue: Skeletal Muscle
C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Troponin I Skeletal Protein
abx060758-100ug 100 ug
EUR 1574

Troponin T Skeletal Protein
abx060767-05mg 0.5 mg
EUR 1713

anti-fast skeletal Myosin
YF-PA13308 50 ug
EUR 363
Description: Mouse polyclonal to fast skeletal Myosin

anti-fast skeletal Myosin
YF-PA24194 50 ul
EUR 334
Description: Mouse polyclonal to fast skeletal Myosin

Skeletal Muscle Cytoplasmic Lysate
XBL-10851 0.1 mg
EUR 227.75
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Skeletal Muscle Membrane Lysate
XBL-10852 0.1 mg
EUR 516.5
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
4-SEC773Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Ryanodine Receptor 1, Skeletal ELISA Kit (RYR1)
RK03928 96 Tests
EUR 521

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-48Tests 48 Tests
EUR 557

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-96Tests 96 Tests
EUR 775

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
DLR-RYR1-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
DLR-RYR1-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) CLIA Kit
20-abx493893
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Slow Skeletal Troponin I (TNNI1) CLIA Kit
20-abx494152
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Fast Skeletal Troponin I (TNNI2) CLIA Kit
20-abx494155
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Slow Skeletal Troponin T (TNNT1) CLIA Kit
20-abx494158
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
20-abx156055
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-48Tests 48 Tests
EUR 583

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-96Tests 96 Tests
EUR 811

Rat Ryanodine Receptor 1, Skeletal(RYR1)ELISA Kit
QY-E10032 96T
EUR 361

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Genomic DNA from Rat Normal Tissue: Skeletal Muscle
D1434171 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Skeletal Muscle
R1434171-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Cancer MicroRNA qPCR Array
RA610A-1 10 profiles
EUR 1172

Mouse Skeletal Muscle Tissue Lysate
LYSATE0021 200ug
EUR 150
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

? skeletal muscle actin Monoclonal Antibody
EM1149-100ul 100ul
EUR 279
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

? skeletal muscle actin Monoclonal Antibody
EM1149-50ul 50ul
EUR 207
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

?-Skeletal Muscle Actin Polyclonal Antibody
EA047-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

?-Skeletal Muscle Actin Polyclonal Antibody
EA047-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

?-Skeletal Muscle Actin Polyclonal Antibody
ES1176-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA

?-Skeletal Muscle Actin Polyclonal Antibody
ES1176-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA

alpha Actin (skeletal muscle) Antibody
35512-100ul 100ul
EUR 390

Rabbit Skeletal Muscle Troponin-C
7-06205 1mg Ask for price

Rabbit Skeletal Muscle Troponin-C
7-06206 5mg Ask for price

Rabbit Skeletal Muscle Troponin-C
7-06207 10mg Ask for price

Troponin I Skeletal Muscle Antibody
abx020870-1mg 1 mg
EUR 815

Troponin I Skeletal Muscle Antibody
abx020871-1mg 1 mg
EUR 815

Anti-fast skeletal Myosin (2D9)
YF-MA10603 100 ug
EUR 363
Description: Mouse monoclonal to fast skeletal Myosin

Anti-fast skeletal Myosin (3H3)
YF-MA18264 100 ug
EUR 363
Description: Mouse monoclonal to fast skeletal Myosin

Anti-fast skeletal Myosin (1B22)
YF-MA19700 100 ug
EUR 363
Description: Mouse monoclonal to fast skeletal Myosin

U2AF1 mutant are preferentially killed by therapy with the compound. Altogether, our outcomes spotlight the potential of trapping early spliceosome meeting as an efficient pharmacological means to manipulate pre-mRNA splicing. By extension, we propose that stabilizing meeting intermediates could supply a helpful method for small-molecule inhibition of macromolecular machines. The software of non-viral programs for delivering genes to cells is changing into a really fascinating difficulty, particularly in the therapy of neoplasms akin to Breast Cancer (BC). Polymer-based non-viral programs are protected and possible gene carriers to be used in focused most cancers remedy. SALL4 gene encodes a transcription issue and is overexpressed in some cancers.