A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

by

Enterohemorrhagic Escherichia coli serotype O157:H7 (O157) is a crucial, foodborne, human intestinal pathogen that causes extreme acute hemorrhagic diarrhea, belly cramping, and even loss of life. Small RNAs (sRNAs) are noncoding regulatory molecules that sense environmental adjustments and set off numerous virulence-related signaling pathways; nonetheless, few such sRNAs have been recognized in O157. Here, we report a novel sRNA, EsrF that senses excessive ammonium concentrations in the colon and enhances O157 pathogenicity by selling bacterial motility and adhesion to host cells.

Specifically, EsrF was discovered to instantly work together with the 5′ untranslated areas of the flagellar biosynthetic gene, flhB, mRNA and improve its abundance, thereby upregulating expression of important flagellar genes, together with flhD, flhC, fliA, and fliC, main to elevated O157 motility and virulence. Meanwhile, an toddler rabbit mannequin of O157 an infection confirmed that deletion of esrF and flhB considerably attenuates O157 pathogenicity.

Furthermore, NtrC-the response regulator of the NtrC/B two-component system-was discovered to exert direct, damaging regulation of esrF expression. Meanwhile, excessive ammonium concentrations in the colon launch the inhibitory impact of NtrC on esrF, thereby enhancing its expression and subsequently selling bacterial colonization in the host colon. Our work reveals a novel, sRNA-centered, virulence-related signaling pathway in O157 that senses excessive ammonium concentrations. These findings present novel insights for future analysis on O157 pathogenesis and focused therapy methods.

The course of by which micro organism sense environmental cues to regulate their virulence is advanced. Several research have centered on regulating the expression of the locus of enterocyte effacement pathogenicity island in the everyday intestine pathogenic bacterium, O157. However, few investigations have addressed the regulation of different virulence elements in response to intestinal indicators. In this research, we report our discovery of a novel O157 sRNA, EsrF, and show that it contributed to bacterial motility and virulence in vitro and in vivo by the regulation of bacterial flagellar synthesis.

Furthermore, we present that top ammonium concentrations in the colon induced esrF expression to promote bacterial virulence by releasing the repression of esrF by NtrC. This research highlights the significance of sRNA in regulating the motility and pathogenicity of O157.  In this research, carboxyalkylated-PEI25 (25 kDa) was used to ship plasmids expressing SALL4-siRNA into MCF-7 cells. DLS and AFM had been utilized to decide the dimensions of nanoparticles. The MTT technique was used to assess cytotoxicity, and the effectivity of transfection was confirmed each qualitatively and quantitatively. Finally, the impact of silencing SALL4 was investigated on the migration of MCF7 cells utilizing the scratch check.

Polyploidy-associated paramutation in Arabidopsis is set by small RNAs, temperature, and allele construction

Paramutation is a kind of non-Mendelian inheritance in which the expression of a paramutable allele adjustments when it encounters a paramutagenic allele. This change in expression of the paramutable alleles is stably inherited even after segregation of each alleles. While the invention of paramutation and research of its underlying mechanism had been made with alleles that change plant pigmentation, paramutation-like phenomena are recognized to modulate the expression of different traits and in different eukaryotes, and many instances have most likely gone undetected.

It is probably going that epigenetic mechanisms are accountable for the phenomenon, as paramutation types epialleles, genes with similar sequences however totally different expression states. This might account for the intergenerational inheritance of the paramutated allele, offering profound proof that triggered epigenetic adjustments will be maintained over generations. Here, we use a case of paramutation that impacts a transgenic choice reporter gene in tetraploid Arabidopsis thaliana. Our information counsel that differing types of small RNA are derived from paramutable and paramutagenic epialleles.

In addition, deletion of a repeat inside the epiallele adjustments its paramutability. Further, the temperature through the progress of the epiallelic hybrids determines the diploma and timing of the allelic interplay. The information additional make it believable why paramutation in this method turns into evident solely in the segregating F2 inhabitants of tetraploid vegetation containing each epialleles. In abstract, the outcomes help a mannequin for polyploidy-associated paramutation, with similarities in addition to distinctions from different instances of paramutation.

A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium

A artificial small molecule stalls pre-mRNA splicing by selling an early-stage U2AF2-RNA advanced

Dysregulated pre-mRNA splicing is an rising Achilles heel of cancers and myelodysplasias. To develop the at present restricted portfolio of small-molecule drug leads, we screened for chemical modulators of the U2AF advanced, which nucleates spliceosome meeting and is mutated in myelodysplasias. A hit compound particularly enhances RNA binding by a U2AF2 subunit. Remarkably, the compound inhibits splicing of consultant substrates and stalls spliceosome meeting on the stage of U2AF perform. Computational docking, along with structure-guided mutagenesis, signifies that the compound bridges the tandem U2AF2 RNA recognition motifs through hydrophobic and electrostatic moieties. Cells expressing a cancer-associated

Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) Antibody
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ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE101020-5platesof96wells 5 plates of 96 wells
EUR 2115
  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE101020-96T 96T
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE30009-5platesof96wells 5 plates of 96 wells
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
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ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-5platesof96wells 5 plates of 96 wells
EUR 2115
  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
KTE70102-96T 96T
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  • Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase
  • TnT, which contains the bindi
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5 1
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Total Protein Extraction Kit for Muscles (50 tests)
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Rat Skeletal Muscle Tissue Lysate
LYSATE0010 200ug
EUR 150
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KS341025 1 kit
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RAL-1467 1 mg
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Myosin antibody (skeletal)
10R-7875 100 ug
EUR 322
Description: Mouse monoclonal Myosin antibody

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10R-7876 100 ug
EUR 322
Description: Mouse monoclonal Myosin antibody

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  • Category: MicroRNA Tools

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Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Skeletal muscle Actinin Alpha ELISA kit
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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Troponin I Skeletal Protein
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anti-fast skeletal Myosin
YF-PA13308 50 ug
EUR 363
Description: Mouse polyclonal to fast skeletal Myosin

anti-fast skeletal Myosin
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EUR 334
Description: Mouse polyclonal to fast skeletal Myosin

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  • Product category: Molecular Biology Kits/RNA - microRNA Extraction/Multipurpose

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Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
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Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
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  • Should the Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
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EUR 282
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Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
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  • 10 × 96 tests
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  • 10 × 96 tests
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  • 10 × 96 tests
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Rat Slow Skeletal Troponin T (TNNT1) CLIA Kit
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  • EUR 8569.00
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Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit
E02A1218-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Actin Alpha 1, Skeletal Muscle ELISA kit
E02A0391-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ryanodine Receptor 1, Skeletal(RYR1)ELISA Kit
QY-E10032 96T
EUR 361

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
E02M0028-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Rat Normal Tissue: Skeletal Muscle
R1434171-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay:
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay:
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay:
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
SEC773Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay:
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
4-SEC773Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Ryanodine Receptor 1, Skeletal elisa. Alternative names of the recognized antigen: CCO
  • MHS
  • MHS1
  • RYDR
  • RYR
  • SKRR
  • Central Core Disease Of Muscle
  • Skeletal muscle calcium release channel
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Ryanodine Receptor 1, Skeletal ELISA Kit (RYR1)
RK03928 96 Tests
EUR 521

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-48Tests 48 Tests
EUR 583

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RDR-RYR1-Ra-96Tests 96 Tests
EUR 811

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-48Tests 48 Tests
EUR 557

Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
RD-RYR1-Ra-96Tests 96 Tests
EUR 775

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit
CSB-EL024011RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle (TNNI1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit
1-CSB-EL024011RA
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle(TNNI1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
CSB-EL024012RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle (TNNI2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
1-CSB-EL024012RA
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle(TNNI2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
CSB-EL024015RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle (TNNT1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
1-CSB-EL024015RA
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle(TNNT1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
CSB-EL024017RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle (TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
1-CSB-EL024017RA
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle(TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
DLR-ACTa1-Ra-48T 48T
EUR 528
  • Should the Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
DLR-ACTa1-Ra-96T 96T
EUR 690
  • Should the Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium
9-25073 5 x 100 ml Ask for price

Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
20-abx156169
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit
20-abx156170
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit
20-abx156172
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Troponin I Type 1, Slow Skeletal (TNNI1) Protein
20-abx069487
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Rat Troponin I Type 2, Fast Skeletal (TNNI2) Protein
20-abx069492
  • EUR 676.00
  • EUR 272.00
  • EUR 2068.00
  • EUR 801.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Rat Troponin T Type 1, Slow Skeletal (TNNT1) Protein
20-abx069498
  • EUR 718.00
  • EUR 286.00
  • EUR 2221.00
  • EUR 857.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit
abx256153-96tests 96 tests
EUR 746
  • Shipped within 5-12 working days.

Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
abx256154-96tests 96 tests
EUR 746
  • Shipped within 5-12 working days.

U2AF1 mutant are preferentially killed by therapy with the compound. Altogether, our outcomes spotlight the potential of trapping early spliceosome meeting as an efficient pharmacological means to manipulate pre-mRNA splicing. By extension, we propose that stabilizing meeting intermediates could supply a helpful method for small-molecule inhibition of macromolecular machines. The software of non-viral programs for delivering genes to cells is changing into a really fascinating difficulty, particularly in the therapy of neoplasms akin to Breast Cancer (BC). Polymer-based non-viral programs are protected and possible gene carriers to be used in focused most cancers remedy. SALL4 gene encodes a transcription issue and is overexpressed in some cancers.